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Image Search Results
Journal: MethodsX
Article Title: Design and construction of a low-cost nose poke system for rodents
doi: 10.1016/j.mex.2016.04.002
Figure Lengend Snippet: Quantification of dopamine neuron optogenetic self-stimulation. Validation of the system through dopamine self-stimulation in DAT-CRE transgenic mice. A) Infection site and TH counter stain verifying infection and dopamine selectivity of ChR2-YFP. Scale bar 1 mm. B) Magnified view of the VTA area showing overlap of TH staining and ChR2-YFP expression. Scale bar 100 μm. C) Example track plot of the first and third sessions of a single mouse overlaid with a normalized heatmap illustrating the distribution of time spent on the platform. D) Example image of a mouse nose poking in the active port. E) Quantification of the nose pokes in the active and inactive ports over time (two-way Anova repeated measures, effect across days F(5,20) = 19.76; p < 0.0001, effect between ports F(1,4) = 115.98; p = 0.0004). F) Quantification of the time spent near the active and inactive ports on the first day (2392 ± 102.3SEM and 308 ± 55.87SEM; p < 0.0001 t -test) and the third day (3205 ± 216.5SEM and 59 ± 29.74SEM; p = 0.0001 t -test).
Article Snippet: To test the functionality of our system we decided to use
Techniques: Biomarker Discovery, Transgenic Assay, Infection, Staining, Expressing